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Fig. 8 | Molecular Cancer

Fig. 8

From: A novel peptide encoded by N6-methyladenosine modified circMAP3K4 prevents apoptosis in hepatocellular carcinoma

Fig. 8

CircMAP3K4-455aa was ubiquitinated by MIB1 and degraded by the ubiquitin–proteasome pathway. A Representative confocal images showing the cytoplasmic location of circMAP3K4-455aa. B The half-life of circMAP3K4-455aa protein after treatment with cycloheximide. C Western blot assay examining circMAP3K4-455aa expression levels after treatment with MG132, NH4Cl, or 3-Methyladenine. D, E. Co-IP (D) and confocal colocalization (E) verified circMAP3K4-455aa interaction with MIB1. F Western blot detection of circMAP3K4-455aa after MIB1 knockdown (left panel) and MIB overexpression (right panel). G In vivo ubiquitylation assay assessing circMAP3K4-455aa ubiquitination after MIB1 knockdown. H Detection of the circMAP3K4 half-life after decreasing MIB1 expression. CHX, Cycloheximide; cMAP, circMAP3K4; 3-MA, 3-Methyladenine; IP, Immunoprecipitation

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