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Fig. 2 | Molecular Cancer

Fig. 2

From: Long non-coding RNA LINC00680 functions as a ceRNA to promote esophageal squamous cell carcinoma progression through the miR-423-5p/PAK6 axis

Fig. 2

LINC00680 promotes cell proliferation, colony formation, migration, and invasion in vitro and tumor growth in vivo. a, b, g, i, k KYSE510 cells were transfected with negative control siRNA (si NC) or two independent siRNAs targeting LINC00680 (si LINC00680 #1 and si LINC00680 #2) followed by RT-qPCR analysis (a), cell proliferation assay (b), colony formation assay (g), wound healing assay (i), and transwell assay (k). Scale bar: 50 µm. h, j, l Quantification of the number of colonies (h), the percentage of wound area (j), and the number of invasive cells (l) as shown in (g), (i), and (k), respectively. c Schematic diagram of the chromosomal location of LINC00680 and the six isoforms confirmed by RACE and PCR assays. d cDNA from KYSE140 cells were subjected to 5’ and 3’ RACE assays to detect the full sequence of LINC00680. PCR products were separated by DNA agarose gel. The six isoforms were indicated by arrows. DNA marker was shown on the left. Sanger sequencing results of the 5’ and 3’ end from PCR products were shown at the bottom, detailed sequencing results could be found in Additional file 4: Table S3. m, n, o, q, s KYSE150 cells were transfected with control vector or vector expressing LINC00680 followed by RT-qPCR analysis (m), cell proliferation assay (n), colony formation assay (o), wound healing assay (q), and transwell assay (s). Scale bar: 50 µm. p, r, t Quantification of the number of colonies (p), the percentage of wound area (r), and the number of invasive cells (t) as shown in (o), (q), and (s), respectively. u KYSE510 cells stably transfected with negative control shRNA (sh NC) or two independent shRNAs targeting LINC00680 (sh LINC00680 #1 and sh LINC00680 #2) were subjected to RT-qPCR analysis. v Cells as described in (u) were subcutaneously injected into nude mice (n = 5 in each group), and images of excised tumors were shown. w The average of the weight of tumors as shown in (v). x The growth curves of tumors as shown in (v). The experiments were repeated for three times, and representative data is shown with mean ± standard deviation (SD), *P < 0.05, **P < 0.01, ***P < 0.001

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