PAK6 promotes the malignant behaviors in ESCC cells. a, b, c, d, e, g, i, k, m, o KYSE510 and KYSE140 cells were transfected with negative control siRNA (si NC) or siRNA targeting PAK6 (si PAK6 #1 and si PAK6 #2) followed by RT-qPCR analysis (a), immunoblotting analysis (b), cell proliferation assay (c-d), colony formation assay (e, g), wound healing assay (i, k), and transwell assay (m, o). Scale bar: 50 µm. f, h, j, l, n, p Quantification of the number of colonies (f, h), the percentage of wound area (j, l), and the number of invasive cells (n, p). q, r, s, t, v, x KYSE150 cells were transfected with control vector or vector expressing PAK6 followed by RT-qPCR analysis (q), immunoblotting analysis (r), cell proliferation assay (s), colony formation assay (t), wound healing assay (v), and transwell assay (x). Scale bar: 50 µm. u, w, y Quantification of the number of colonies (u), the percentage of wound area (w), and the number of invasive cells (y) as shown in (t), (v), and (x), respectively. The experiments were repeated for three times, and representative data is shown with mean ± standard deviation (SD), *P < 0.05, **P < 0.01, ***P < 0.001