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Fig. 2 | Molecular Cancer

Fig. 2

From: KMT2C methyltransferase domain regulated INK4A expression suppresses prostate cancer metastasis

Fig. 2

Loss of the Kmt2c SET domain exacerbates prostate cancer tumorigenesis in Pten-null mice. a Scheme of the constructs allowing for the double deletion of Kmt2c (top) and Pten (bottom) in combination with expression of Cre recombinase under the control of the androgen-regulated probasin promoter. Mutated transcripts expressed specifically in the prostate epithelium after recombination are depicted on the right-hand side. b Representative pictures of prostates of wild type, Pten∆/∆, Pten∆/∆Kmt2cSET∆/+ and Pten∆/∆Kmt2cSET∆/∆ mice resected at 19 weeks p.p. Scale bar: 10 mm. c Quantification of prostate tissue weight of wild type, Pten∆/∆, Pten∆/∆Kmt2cSET∆/+ and Pten∆/∆Kmt2cSET∆/∆ prostates at 19 weeks p.p. (n ≥ 13) d Percentage of cystic fluid per total tumour weight (solid tissue and fluid) of Pten∆/∆, Pten∆/∆Kmt2cSET∆/+ and Pten∆/∆Kmt2cSET∆/∆ prostates at 19 weeks p.p. (n ≥ 13) e Representative pictures of H&E (left panel) and Ki-67 (right panel) IHC staining of wild type, Pten∆/∆, Pten∆/∆Kmt2cSET∆/+ and Pten∆/∆Kmt2cSET∆/∆ prostates at 19 weeks p.p., Scale bars: 100 µm. f Quantification of cells positive for Ki-67 using QuPath software. (n ≥ 8) g Kaplan Meier cumulative survival analysis of wild type, Kmt2cSET∆/∆, Pten∆/∆, Pten∆/∆Kmt2cSET∆/+ and Pten∆/∆Kmt2cSET∆/∆ mice (n ≥ 5). Values next to the dotted lines at the x-axis of the graph indicate the median life expectancy. P values were determined by log-rank (Mantel-Cox) tests. (c, d, f) Individual biological replicates are shown. Data are plotted as mean ± standard deviation, and P values were determined by ordinary one-way ANOVA with Tukey’s multiple comparisons test

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