Fig. 3

KMT2C methyltransferase deficiency triggers metastatic dissemination of prostate cancer. a H&E stains of urinary tract obstruction by invasive PCa in a moribund Pten^∆/∆Kmt2c^SET∆/∆ mouse at 39 weeks p.p. at low (top) and high (bottom) magnification. Scale bars: 200 µm. T, tumour; U, urethra. b H&E-stained sections and IHC analysis of the androgen receptor (AR) and keratin 8 (KRT8) of tumour dissemination into lumbar lymph nodes, blood vasculature and lungs. Depicted material was derived from two moribund animals at 46- and 48-weeks p.p. Scale bars: 100 µm. LN, lymph node; L, lung; E, endothelial lumen; M, metastasis. c-d Percentage of wild type, Pten^∆/∆, Pten^∆/∆Kmt2c^SET∆/+ and Pten^∆/∆Kmt2c^SET∆/∆ mice presenting with lymph node (c) and lung (d) metastasis at the time of death. The number above the bar indicates metastasis-bearing/total number of analysed mice (n ≥ 5). e Kaplan Meier analysis depicting wild type, Pten^∆/∆, Pten^∆/∆Kmt2c^SET∆/+ and Pten^∆/∆Kmt2c^SET∆/∆ mice having developed metastasis at the time of death (n ≥ 5). Mice that did not present with metastasis at the time of death are shown as censored events. The value below the dotted line indicates the median metastasis-free survival of Pten^∆/∆Kmt2c^SET∆/∆ mice. The P value was determined by a log-rank (Mantel-Cox) test