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Fig. 3 | Molecular Cancer

Fig. 3

From: Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression

Fig. 3

EGFR activation sustains ERRα re-expression. a Western blot assay showed p-EGFR, EGFR and ERRα proteins level after treatment with gefitinib or osimertinib. b Western blot assay showed the proteins level after treatment with osimertinib or lapatinib. c PC-9, PC-9/GR, H1975 and PC-9/OR cells were treated with 1 μM gefitinib or 0.1 μM osimertinib for 6 h. RT-qPCR showed ERRα mRNA level. d PC-9/GR, H1975, PC-9/OR cells were treated with 1 μM osimertinib or 1 μM lapatinib for 6 h. RT-qPCR showed ERRα mRNA level. e Cells were transfected with NC or siEGFR, after 48 h, cells were harvested and the proteins level were detected by Western blot assay. Data are expressed as mean ± SEM (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001 in a, b, c, d and e

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