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Fig. 4 | Molecular Cancer

Fig. 4

From: LSD1 deletion decreases exosomal PD-L1 and restores T-cell response in gastric cancer

Fig. 4

LSD1 deletion downregulates exosomal PD-L1 in GC. a SEM images of purified exosomes from BGC-823 cells in the presence or absence of LSD1 (B-EXO and B KO-EXO, respectively, for short). Scale bar = 100 nm. b Size distribution of the BGC-823 and LSD1 deleted BGC-823 cell-derived exosomes analyzed by NTA. c Analysis of the fractions collected after sucrose gradient centrifugation at 100,000 g; pellets obtained from ultracentrifugation of BGC-823 cells supernatants. d Expression of PD-L1 in a panel of GC cell-derived exosomes. e Expression of PD-L1 in BGC-823 and MGC-803 and their corresponding LSD1-deleted cell-derived exosomes. f Expression of PD-L1 in B-EXO from cells treated with or without GSK2879552 and ORY1001. g Expression of PD-L1 in B-EXO & B KO-EXO conjugated with 5 μm beads analyzed by flow cytometry. h Expression of PD-L1 in exosomes from MGC-803 cells (M-EXO) and MGC-803 LSD1 KO cells (M KO-EXO) conjugated with 5 μm beads analyzed by flow cytometry. i Concentration of B-EXO and B KO-EXO. j Expression of PD-L1 in BGC-823 or BGC-823 LSD1 KO cells treated with or without 10 μM GW4869 for 24 h. The data are presented as the mean ± SD; n = 3; n.s, no significance, *P < 0.05; two-tailed unpaired Student’s t-test

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