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Fig. 5 | Molecular Cancer

Fig. 5

From: LSD1 deletion decreases exosomal PD-L1 and restores T-cell response in gastric cancer

Fig. 5

LSD1 deletion reverses the direct T-cell response suppression of GC-derived exosomal PD-L1. a Representative confocal images of PKH26 stained B-EXO and B KO-EXO with wells coated with recombinant PD-1. Scale bar = 50 μm. b SEM images of the binding of T cells to B-EXO/M-EXO or B KO-EXO/M KO-EXO. Scale bar = 1 μm. c Expression of CD69 in CD8 + T cells incubated with B-EXO or B KO-EXO, respectively. NT indicates no treatment with anti-CD3/CD28 beads. d T-cell proliferation in anti-CD3/CD28-stimulated PBMC incubated with B-EXO or B KO-EXO. e and f Cell survival of BGC-823 cells co-incubated with anti-CD3/CD28 beads-activated PBMC in the presence of B-EXO or B KO-EXO. Representative images are shown on the left (e), quantification is provided on the right (f). g-i ELISA analysis of IL-2 (g), IFN-γ (h), and TNFα (i) concentration in anti-CD3/CD28-stimulated PBMC in the presence of B-EXO or B KO-EXO. The data are presented as the mean ± SD; n = 3; n.s, no significance, * P < 0.05; ** P < 0.01; *** P < 0.001; two-tailed unpaired Student’s t-test

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