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Fig. 2 | Molecular Cancer

Fig. 2

From: The N6-methyladenosine modification of circALG1 promotes the metastasis of colorectal cancer mediated by the miR-342-5p/PGF signalling pathway

Fig. 2

Characterization of circALG1 in CRC cells. A The expression of circALG1 was detected by qRT-PCR followed by Sanger sequencing. The arrows represent different primers (left). The RT-PCR products from different primers were detected by agarose gel electrophoresis (right). B SW480 cells were treated with RNase R, and the expression of circALG1 and its maternal gene ALG1 was then assessed by qRT-PCR. C SW480 cells were treated with actinomycin, and the stability of circALG1 was then assessed by qRT-PCR. D Analysis of the subcellular localization of circALG1 using a nucleus-cytoplasm separation assay. E Subcellular localization of circALG1 detected by FISH. The results are presented as the mean ± s.d. and are representative of at least 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, #p > 0.05

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Molecular Cancer

ISSN: 1476-4598