Fig. 4
From: CRISPR/Cas9 and next generation sequencing in the personalized treatment of Cancer
Represents the mechanism of CRISPR/Cas9 mediated CAR T cell immunotherapy: The T cells from the patients are removed and genetically modified by CRISPR/Cas9 mediated knock-in/knock-out mechanism, giving rise to the Chimeric Antigen Receptor T cells that contain an intracellular chimeric signalling domain that can activate T cells and an external single-chain variable segment that can recognise tumour antigens precisely. These CRISPR/Cas9 edited CAR T cells are again introduced into the host as treatment