Fig. 2
HIF-1α combines with WSB1 promoter to increase the expression of circWSB1 under hypoxia. a and b Western blot and qRT-PCR analyses of HIF1α protein level and circWSB1 expression in MCF-7 cells treated with indicated concentration of O2 for 24 h (a) or different periods of time under 1% O2 (b). c Western blot and qRT-PCR analyses showing the HIF1α protein level and circWSB1 expression in MCF-7 cells cultured under indicated conditions. d qRT-PCR analyses to examine the relative expression of circWSB1 in MCF-7 cells treated with different concentration of CoCl2 or 400 μM CoCl2 for indicated periods of time. e and f Western blot and qRT-PCR analyses of the HIF1α protein level and circWSB1 expression in MCF-7 cells after knockdown (e) or overexpression (f) of HIF1α. g Schematic illustration of putative HREs in the promoter region of WSB1 gene. h and i Luciferase reporter assays for the relative activity of the putative HREs of WSB1 in MCF-7 cells treated with indicated conditions. j ChIP assays were performed with anti-HIF1α or IgG control in MCF-7 cells cultured under hypoxia. Data are shown as mean ± SD and representative of three independent experiments in (a-f) and (h-j). *P < 0.05, **P < 0.01, ***P < 0.001