Fig. 7

CircWSB1 facilitates BC cell growth through inhibiting the interaction between USP10 and p53. a Co-IP assays were conducted with anti-USP10 in hypoxic MCF-7 cells transfected with indicated plasmids. b Ubiquitination levels of p53 were detected in MG132-treated MCF-7 and MDA-MB-453 cells co-transfected with indicated vectors or siRNAs under hypoxia. c Western blot analyses of MCF-7 cells after transfected with indicated vectors under hypoxia. d CCK-8 assays of MCF-7 cells under hypoxic conditions. e–h Flow cytometric cell cycle assays (e, f) and apoptosis analyses (g, h) of hypoxic MCF-7 cells treated with indicated siRNAs. i Representative bioluminescence imaging of tumor-bearing mice injected with indicated stable MCF-7 cells. j Photograph of xenograft tumors removed from each nude mouse (n = 5). k The weight of each group of xenograft tumor was calculated. l Growth curves of xenograft tumors of each group of nude mice were minored and measured once a week. m IHC staining to determine the expression of Ki67 and p53 in xenograft tumors, scale bar, 100 μm. n Kaplan–Meier survival curves of the tumor-bearing mice (n = 10). o Schematic diagram illustrating the generation of circWSB1 and the mechanisms how circWSB1 promotes the progression of BC under hypoxia. Data are showed as mean ± SD or representative of three independent experiments in (c-h). **P < 0.01, ***P < 0.001