Fig. 3

Effects of circ-MTCL1 on proliferation, invasion and migration through C1QBP in LSCC cells. a, The expression level of circMTCL1 was identified after overexpressing or knocking down circMTCL1 by qRT-PCR assays. Values are the mean ± s.d. of n = 3 independent experiments. b, Western blot assays were performed to evaluate the expression levels of β-catenin and p-β-catenin upon circMTCL1 silencing or overexpressing in TU212 and LCC cells. c, The cytoplasmic and nuclear accumulation of β-catenin and p-β-catenin were determined after ectopic or knockdown circMTCL1 by Wertern blot assays. d, The proliferation ability was measured upon circMTCL1 overexpressing or silencing in TU212 and LCC cells by EdU assays. Scale bars = 50 μm. e, Wound healing assays were performed to identify the cell motility upon circMTCL1 silencing or overexpressing in TU212 and LCC cells. f, The migrated cell numbers were determined after ectopic or knockdown circMTCL1 in TU212 and LCC cells. g,Western blot assays detected the expression of β-catenin and p-β-catenin in TU212 and LCC cells co-transfected circMTCL1 and C1QBP. h, CircMTCL1 rescued the proliferation ability after co-transfected circMTCL1 and C1QBP. i, Overexpressing circMTCL1 rescued the vertically migrated and invasive ability after silencing C1QBP. Scale bars = 500 μm. J, Overexpressing circMTCL1 rescued the laterally migrated ability after silencing C1QBP. Scale bars = 500 μm. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001