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Fig. 2 | Molecular Cancer

Fig. 2

From: The m6A demethylase ALKBH5 promotes tumor progression by inhibiting RIG-I expression and interferon alpha production through the IKKε/TBK1/IRF3 pathway in head and neck squamous cell carcinoma

Fig. 2

ALKBH5 inhibition decreases cell proliferation in vitro and tumor growth in vivo. a The knockdown efficiency was detected using immunoblotting after siRNA transfection for 48 h. b, c Cell proliferation and colony formation assays were performed. d The EdU assay was performed after siRNA transfection for 48 h. e The cell cycle distribution was analyzed after transfection for 48 h using flow cytometry (PI staining). f Gene set enrichment analysis (GSEA) plots evaluating the alterations in the cell cycle and DNA replication using RNA sequence data after ALKBH5 knockdown in the Cal27 cell line. NES, normalized enrichment score. g The transfection efficiency was detected after lentivirus transduction of ALKBH5 in Cal27 cells. h Tumor growth was measured once a week in tumor-bearing nude mice stably transfected with Cal27 cells. i The tumors were removed, and the tumor weight was analyzed after the mice were killed. j, k TUNEL staining and KI-67 staining were performed in tumor tissues from xenografts. Scale bar: 100 μm, Data information: Data are presented as the mean ± S.D. One-way ANOVA; *P < 0.05, **P < 0.01

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