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Fig. 3 | Molecular Cancer

Fig. 3

From: The m6A demethylase ALKBH5 promotes tumor progression by inhibiting RIG-I expression and interferon alpha production through the IKKε/TBK1/IRF3 pathway in head and neck squamous cell carcinoma

Fig. 3

Characterization of m6A modification and analysis of downstream targets of ALKBH5 in HNSCC. a, b Dot blot and m6A RNA methylation quantification analyses were performed after ALKBH5-specific siRNA transfection for 48 h. c Venn diagrams showing 171 genes with differential expression genes and differential m6A-methylation in si-ALKBH5 cells compared with control Cal27 cells (top). A total of 171 common differential genes were classified according to the level of mRNA and m6A methylation (bottom). d Predominant consensus motifs identified by HOMER with m6A-seq peaks in Cal27 cells with or without ALKBH5 knockdown. e, f Number of m6A peaks and m6A-modified genes identified in m6A-seq in si-ALKBH5 and si-scr cells. Unique m6A genes containing no common m6A peaks. g Graphs of the m6A peak distribution showing the proportion of total m6A peaks in the indicated regions (top) and the unique m6A peak distribution after ALKBH5 knockdown (bottom). h Heatmap showing the expression profile of differentially methylated genes after ALKBH5 knockdown in Cal27 cells with two replicates. i The GO terms are visualized in a chord plot. *P < 0.05, **P < 0.01

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