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Fig. 1 | Molecular Cancer

Fig. 1

From: An EHMT2/NFYA-ALDH2 signaling axis modulates the RAF pathway to regulate paclitaxel resistance in lung cancer

Fig. 1

Identification of ALDH2 as a paclitaxel resistance-related gene in NSCLC. A Bubble diagram of KEGG pathway analysis based on the differentially expressed genes in NCI-H460/PTX cells compared with NCI-H460 cells (three biological replicates for each condition). The diagram shows enriched pathways. B Colony formation assay, tumor sphere formation assay and Transwell migration assay in NCI-H460 and NCI-H460/PTX cells. The photographs were taken at magnifications of × 200. Scale bar = 50 μm. C The protein expression levels of the stem cell transcription factors Sox2, Nanog, and Oct4 in NSCLC and NSCLC/PTX cells. D ALDH activity was determined in NSCLC and NSCLC/PTX cells. Cells were labeled with Aldefluor with or without the ALDH inhibitor DEAB and analyzed by flow cytometry. E The protein expression levels of ALDH subtypes in NSCLC and NSCLC/PTX cells. F The relationship between ALDH2 expression level and PTX resistance in lung cancer cells. G Correlation of the expression of ALDH2 protein with overall survival. ***p < 0.001, as compared to parental cells

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