Fig. 5

Knockdown of ALDH2 inhibits the RAS/RAF pathway in NSCLC/PTX cells. A Volcano plot of gene microarray data illustrating upregulated and downregulated genes of interest (fold change > 2 and p < 0.05) in NCI-H460/PTX cells transfected with ALDH2 shRNA compared with scramble (three biological replicates for each condition). B Venn diagram showing overlapping genes between upregulated genes in NCI-H460/PTX compared with NCI-H460 and downregulated genes in NCI-H460/PTX transfected with ALDH2 shRNA compared with scramble. C Bubble diagram of KEGG pathway analysis based on the differentially expressed genes in NCI-H460/PTX cells transfected with ALDH2 shRNA compared with scramble. Enriched pathways are shown. D The expression levels of RAS/RAF pathway-related proteins in NSCLC/PTX cells transfected with ALDH2 shRNA or scramble. E MTT assay results showing the efficacy of PTX in ALDH2 knockdown NSCLC/PTX cells transfected with KRAS overexpression (OE) or empty plasmid for 72 h. F MTT assay results showing the efficacy of PTX in ALDH2 knockdown NSCLC/PTX cells transfected with RAF1 overexpression (OE) or empty plasmid for 72 h. ^*p < 0.05, ^**p < 0.01, as compared to the empty group