Skip to main content
Fig. 4 | Molecular Cancer

Fig. 4

From: AP4 suppresses DNA damage, chromosomal instability and senescence via inducing MDC1/Mediator of DNA damage Checkpoint 1 and repressing MIR22HG/miR-22-3p

Fig. 4

MDC1 is directly regulated by AP4. A qPCR analysis of nascent MDC1 mRNA 48 h after AP4 induction by DOX (left panel). qPCR analysis of nascent MDC1 mRNA in untreated cells (right panel). B ChIP-Seq analysis of AP4 occupancy using the UCSC genome browser. C Validation of MDC1 as AP4 direct target via qChIP assay. Cells were treated with DOX for 48 h. Chromatin was enriched by anti-AP4 or anti-rabbit-IgG antibodies. SNAI1 and 16q22 served as positive and negative controls, respectively. D-E qPCR and Western blot analysis of MDC1 in the indicated cell lines 48 h after transfection. F Western blot analysis of MDC1 after AP4 induction by DOX for the indicated durations. G qPCR analysis of nascent MDC1 mRNA after c-MYC induction by DOX for 48 h. H Western blot analysis after c-MYC induction by DOX for the indicated periods. I Model of the AP4/miR-22-3p/MDC1 coherent feed-forward loop. In panels A, C, D and G, the mean + SD (n = 3) is provided with *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001

Back to article page

Molecular Cancer

ISSN: 1476-4598