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Fig. 7 | Molecular Cancer

Fig. 7

From: AP4 suppresses DNA damage, chromosomal instability and senescence via inducing MDC1/Mediator of DNA damage Checkpoint 1 and repressing MIR22HG/miR-22-3p

Fig. 7

MDC1 mediates effects of AP4 on DNA repair. A Detection of MDC1 and γH2AX foci by immunocytochemistry 48 h after silencing MDC1. Scale bars: 20 μm. Foci quantification was performed with Image J software. Nuclei with over 10 foci were considered as positive. The fluorescence intensity was normalized to DAPI. Quantification of 3 fields with 120 cells in total. B MDC1 and γH2AX foci were detected by immunocytochemistry 48 h after ectopic expression of MDC1. Scale bars: 20 μm. Nuclei with over 10 foci were considered as positive. The fluorescence intensity was normalized to DAPI. Quantification of 3 fields with 120 cells in total. C β-gal staining 48 h after silencing or ectopic expression of MDC1, respectively. Quantification of 3 fields with 120 cells in total. Scale bars: 50 μm. MTT assay results 48 h after D silencing MDC1 or E ectopic expression of MDC1. Detection of F γH2AX foci by immunocytochemistry, G β-gal staining and H comet assay in DLD-1 AP4 WT1 cells 48 h after transfection. MDC1-HA was rendered non-responsive to miR-22-3p by deletion of the MDC1 3'-UTR [28]. Quantification of DNA tail moment in 10 fields with 150 cells in total. The mean + SD is provided with A-E (n = 3), F-G (n = 5) and H (n = 10) with **: p < 0.01, ***: p < 0.001, ****: p < 0.0001

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