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Fig. 2 | Molecular Cancer

Fig. 2

From: Mutations in ALK signaling pathways conferring resistance to ALK inhibitor treatment lead to collateral vulnerabilities in neuroblastoma cells

Fig. 2

NF1 knockout leads to ALK inhibitor resistance in neuroblastoma cell lines. a Knockout of NF1 in different ALK-mutated neuroblastoma cell lines using CRISPR-Cas9 leads to an absence of NF1 protein. b Western blot analysis of total and phosphorylated ERK 1/2 indicates increased RAS/MAPK signaling in NF1 knockout single-cell clones. c and d Cell viability of NF1 knockout clones was assessed during ALK inhibitor treatment with ceritinib or lorlatinib and indicated decreased cell sensitivity; values represent mean ± SD, n = 3

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