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Fig. 6 | Molecular Cancer

Fig. 6

From: Mutations in ALK signaling pathways conferring resistance to ALK inhibitor treatment lead to collateral vulnerabilities in neuroblastoma cells

Fig. 6

NF1 knockout cell line models show increased RAS-MAPK signaling and a weakened ERK-RAF feedback. a Schematic of perturbation experiments performed in NF1 knockout cell lines. b Schematic of computational modeling using STASNet. c Computational modeling of ALK downstream signaling using STASNet. Model paths strength shown as a heatmap based on relative values of scaled parameters with separate scaling for LAN-5 and SH-SY5Y cell lines when those parameter values are found to vary between cell lines. Negative feedback is indicated in blue. NF1 knockout models show a weaker ERK-RAF inhibitory feedback in comparison to the respective parental cell line (red box). d Shown is the log-fold change of phosphorylated MEK level for the parental cell line and NF1 knockout models stimulated with EGF or stimulated with EGF and exposed to MEK inhibitor, compared to an unperturbed control, values represent mean ± standard error of the mean, n = 3

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Molecular Cancer

ISSN: 1476-4598