Fig. 1

The clinical significance of circHMGB2 in NSCLC patients. A The expression of HMGB2-derived circRNAs was measured in 8 pairs of NSCLC tissues and matched normal tissues. B Schematic diagram of circHMGB2 and Sanger sequencing results of the site of circHMGB2 backsplicing. C PCR results of circHMBG2 transcribed with divergent or convergent primers from cDNA and gDNA. D circHMGB2 and HMGB2 in H1703 and H1299 cell lines were detected by qRT–PCR after digestion by RNase. E and F The expression of circHMGB2 was detected by qRT–PCR in 120 pairs of NSCLC tissues and matched normal tissues. G and H The expression of circHMGB2 was analyzed according to tumor diameter (< 2 cm vs. ≥ 2 cm) and lymph node metastasis status (yes vs. no). I The expression of circHMGB2 was analyzed according to TNM stage. J Survival analysis of the recurrence and OS of 120 NSCLC patients divided into groups according to circHMGB2 expression (circHMGB2^high vs. circHMBG2^low) was performed using Kaplan–Meier and log rank analysis. Data are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, *** P < 0.001, ***P < 0.0001, ns: not significant