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Fig. 6 | Molecular Cancer

Fig. 6

From: The circular RNA circHMGB2 drives immunosuppression and anti-PD-1 resistance in lung adenocarcinomas and squamous cell carcinomas via the miR-181a-5p/CARM1 axis

Fig. 6

CircHMGB2 inhibited the type 1 IFN response via CARM1 in NSCLC. A Activation of the STAT1 pathway in A549-circHMGB2 cells after stimulation with IFN-γ for 48 h was verified by western blotting. B STAT1 acetylation in circHMGB2-overexpressing A549 cells or CARM1-KO A549 cells was analyzed by STAT1-IP and anti-acetyl lysine Western blotting. C The effect of CARM1 on the proliferation of A549-circHMGB2 cells stimulated with IFN-γ was measured by CCK-8 assay. D The effect of CARM1 on the apoptosis of A549-HMGB2 cells stimulated with IFN-γ was measured by flow cytometry. E Expression levels of type 1 IFN response-related genes were measured by qRT–PCR after the overexpression of circHMGB2 and the knockout of CARM1 in A549 cells. F and G The relationship between the expression of circHMGB2 and the staining of CARM1, p-STAT1, ISG15 and IFIT1 in 120 NSCLC patients was assessed by IHC and qRT–PCR. Data are presented as the means ± SD; n = 3, **P < 0.01, ***P < 0.001, ns: not significant

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Molecular Cancer

ISSN: 1476-4598