Fig. 6
RhoB reversed hsa-miR-3178-mediated proliferation and gemcitabine resistance in PANC-1-GEM cells. Co-transfection of siRhoB with hsa-miR-3178 inhibitor in PANC-1-GEM cells. A CCK-8 assay for IC50 of gemcitabine in PANC-1-GEM cells with indicated transfection. B The CCK-8 growth curves of indicated cells upon treatment with gemcitabine (150 μmol/L). C The representative fluorescent micrograph and quantification of the EdU staining of indicated cells upon treatment with gemcitabine (150 μmol/L). D Flow cytometry on cell apoptosis in indicated cells exposed to gemcitabine (150 μmol/L) for 72 h. Data are expressed as mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. IC50: half-inhibitory concentration; Mock: untreated cells; INC: Inhibitor negative control; vector: vector-only control; siRhoB: RhoB small interfering RNA; Scr: scramble control; PI: Propidium Iodide; FITC: Fluorescein Isothiocyanate