Fig. 3

Overexpressing circVAPA elevates cell viability, reduces apoptosis of SCLC cells, and accelerates cell cycle progression in vitro. a RT-qPCR analysis of circVAPA and VAPA mRNA expression upon circVAPA overexpression. EV, the empty vector; circVAPA, the circVAPA overexpression plasmid. b Cell viability was assessed by the CellTiter-Glo assay. c Colony formation assay (DMS273) and soft agar colony formation assay (H82) were used to assess cell survival in SCLC cells transfected with indicated plasmid. d The apoptosis rate was analyzed by flow cytometry after overexpressing circVAPA in SCLC cells. e Effects on cell cycle progression analyzed by flow cytometry after circVAPA overexpression. f The expressions of apoptosis-related protein (cleaved-PARP) and cycle-related protein (p21) were detected by Western blot. β-actin was used as an internal reference. C.PARP, cleaved PARP. (All data are presented as the mean ± SD; ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001 by two-tailed Student’s t-test). Three independent assays were performed in the above assays