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Fig. 5 | Molecular Cancer

Fig. 5

From: Single-cell RNA-seq reveals the genesis and heterogeneity of tumor microenvironment in pancreatic undifferentiated carcinoma with osteoclast-like giant-cells

Fig. 5

Heterogeneity of epithelial cells in UCOGCP sample (pca_ai1). A, UMAP showed the clusters learned by Seurat in R, and the mapping of cluster 15 in Fig. 1D. B, Top three markers of each cluster obtained from “FindAllMarkers” function from Seurat package (4.0.4) shown in heatmap. C, Trajectory analysis of the epithelial cells in UCOGCP sample. The mapping of the cluster 13/cluster 15 in Fig. 1D and the pseudotime shown in DDRTree reduction in monocle2 package (2.18.0) in R (4.0.5). D, Mapping of different states in Seurat clusters shown in UMAP. E, The differential expression genes (DEGs) of different branches (different cell fates in C) shown in heatmap. The top GO BP pathways of different clusters in heatmap were listed nearby. F, Top five DE genes determined cell fate in C shown among different states. G, Transcription factor profile of different states shown in heatmap. Transcription factor profile were estimated by pySCENIC (0.11.2)

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