Fig. 5

Restoration of PRMT5 expression reverses phenotype changes induced by miR-331-3p. A-C CCK-8, plate colony-formation and EdU incorporation assays were performed to determine the proliferation abilities of PC-3 and DU145 cells treated with control, oe-miR-331-3p, oe-miR-331-3p + Vector or oe-miR-331-3p + oe-PRMT5. D Image of subcutaneous tumor xenografts in control, oe-miR-331-3p, oe-miR-331-3p + Vector or oe-miR-331-3p + oe-PRMT5 groups. E The tumor growth curves of xenografts were plotted in control, oe-miR-331-3p, oe-miR-331-3p + Vector or oe-miR-331-3p + oe-PRMT5 groups. F Representative IHC staining micrographs of Ki-67 in tumor xenografts were conducted. Scale bar = 20 μm. G and H Wound healing and transwell assays were performed to determine the migratory capabilities of PC-3 and DU145 cells treated with control, oe-miR-331-3p, oe-miR-331-3p + Vector or oe-miR-331-3p + oe-PRMT5. I BALB/c nude mice injected with cells (control, oe-miR-331-3p, oe-miR-331-3p + Vector or oe-miR-331-3p + oe-PRMT5; Five mice per group) via tail vein were imaged at 40 days by in vivo imaging system to evaluate the whole metastasis. J Representative images of H&E staining of lung metastasis loci. Data were showed as mean ± SD. **P < 0.01