Fig. 2

CircSTX6 promotes the proliferation, migration and invasion of PDAC cells in vitro. a-b qRT–PCR analysis of circSTX6 and linear STX6 expression in PANC-1 and CFPAC-1 cells transfected with circSTX6 siRNAs and circSTX6 plasmid. c-f Colony formation assays were performed in PDAC cells transfected with cirSTX6 siRNAs or plasmid. g-h EdU assays of PANC-1 cells were performed to evaluate cell proliferation. Original magnification 400 ×. Scale bar = 50 μm. i-j The growth curves of cells were evaluated by CCK-8 assays after knocking down and overexpressing circSTX6 in PANC-1 cells. k-l Transwell assays in circSTX6-knockdown and circSTX6-overexpressing PANC-1 cells. Original magnification 200 ×. Scale bar = 100 μm. m-n Wound healing assays were used to investigate the migratory ability of circSTX6-overexpressing and circSTX6-knockdown PANC-1 cells. Original magnification 200 ×. Scale bar = 100 μm. (Values are expressed as the means ± SDs; *P < 0.05, **P < 0.01 and ***P < 0.001)