Fig. 5

MiR-449b-5p suppresses the proliferation, migration and invasion of PDAC cells in vitro. a The construction of PDAC cells transfected with miR-449b-5p mimics and miR-449b-5p inhibitor. b-d CCK-8 and colony formation assays of PDAC cells were performed to evaluate cell proliferation. e-h Transwell and wound healing assays were used to investigate the migratory and invasive abilities of PDAC cells transfected with miR-449b-5p mimics and miR-449b-5p inhibitor. (e-h, original magnification 200 ×. Scale bar = 100 μm. Values are expressed as the means ± SDs; *P < 0.05, **P < 0.01 and ***P < 0.001)