Fig. 7

MYH9 is indirectly regulated by circSTX6. a-c The expression levels of MYH9 mRNA (a-b) and protein (c) were significantly upregulated in circSTX6-overexpressing and downregulated in circSTX6 knockdown PDAC cells, and these effects could be reversed by the transfection of miR-449b-5p mimics or inhibitor. d Correlation analysis showed a negative relationship between the levels of miR-449b-5p and MYH9. e Correlation analysis showed a positive correlation between the levels of circSTX6 and MYH9. f MYH9 knockdown and overexpression cell lines were constructed. g-j MYH9 knockdown suppressed and MYH9 overexpression promoted the migratory and invasive abilities of PDAC cells, and these effects could be rescued by the transfection of circSTX6 plasmid or siRNA. Original magnification 200 ×. Scale bar = 100 μm. k-o The results of colony formation and CCK-8 assays revealed that MYH9 knockdown suppressed and MYH9 overexpression promoted proliferative abilities of PDAC cells, and these effects could be reversed by the transfection of circSTX6 plasmid or siRNA. (Values are expressed as the means ± SDs; *P < 0.05, **P < 0.01 and ***P < 0.001)