Fig. 1

CBX2 is overexpressed in leukemia. A Dot plot representation of CBX2 expression in publicly available RNA-seq data from 176 primary human AML samples, 19 human hematopoietic progenitor samples, and 16 differentiated human monocyte and macrophage samples. RPKM values for each dataset were normalized to ACTB levels within each dataset and compared (P < 0.01, Welch’s t-test). B RT-qPCR analysis of CBX2 transcript in primary AML samples (pt) compared to CD34⁺ cells; error bars show SD of three biological replicates (*P < 0.05, **P ≤ 0.01). C WB analysis of CBX2 protein levels in a panel of leukemic cell lines and CD34⁺ cells; ERK1/2 was used as loading control