Fig. 2

Effects of MARCKSL1–2 on the proliferation and apoptosis of DTX-sensitive or -resistant LAD cells under increasing dose of DTX treatment. H1299 cells were transfected with shRNAs targeting MARCKSL1–2, and H1299/DTX cells were transfected with pcDNA/MARCKSL1–2. a-d Colony formation and EdU assays (Scale bar = 100 μm) measured the impact of MARCKSL1–2 inhibition or overexpression on the proliferation capacity of H1299 cells treated with different doses of DTX (0, 5 and 10 μg/L) and H1299/DTX cells treated with different doses of DTX (0, 50 and 100 μg/L). e-h Flow cytometry analyses and TUNEL assays (Scale bar = 100 μm) detected the apoptosis rate of H1299 cells and H1299/DTX cells under different conditions. ^*P < 0.05, ^**P < 0.01. n.s.: no significance