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Fig. 4 | Molecular Cancer

Fig. 4

From: MARCKSL1–2 reverses docetaxel-resistance of lung adenocarcinoma cells by recruiting SUZ12 to suppress HDAC1 and elevate miR-200b

Fig. 4

MARCKSL1–2 interacts with SUZ12 in LAD cells. a-b ChIP assay detected the changes in histone-H3 acetylation level at miR-200b promoter in SPC-A1/H1299 cells under shMARCKSL1–2 suppression and that in SPC-A1/DTX/H1299/DTX cells upon MARCKSL1–2 overexpression. c RNA pull-down assay followed by mass spectrometry analysis examined the proteins binding with MARCKSL1–2. d RNA pull- down plus Western blot detected the existence of SUZ12 in the pull-down complexes of MARCKSL1–2. e-f RIP assays followed by AGE or RT-qPCR examined the enrichment of MARCKSL1–2 in SUZ12 groups. g-h. RT-qPCR and Western blot detected the influence of MARCKSL1–2 on SUZ12 expression in indicated LAD cells. **P < 0.01. n.s.: no significance

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