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Fig. 1 | Molecular Cancer

Fig. 1

From: Circular RNA EIF4G3 suppresses gastric cancer progression through inhibition of β-catenin by promoting δ-catenin ubiquitin degradation and upregulating SIK1

Fig. 1

CircEIF4G3 is downregulated in GC. (A) GEO datasets were downloaded for integrated analyses of differentially expressed circRNAs. The common downregulated circRNAs were listed as indicated. (B) Genomic location of circEIF4G3. CircEIF4G3 was formed by the back-splicing of exons 3–5 of EIF4G3. (C) The backsplice junction site of circEIF4G3 was identified by Sanger sequencing. (D) PCR assay of gDNA and cDNA using divergent and convergent primers of circEIF4G3. (E) The stability of circEIF4G3 and EIF4G3 mRNA was detected by RNase R degradation assay. Data are shown as means ± SD (n = 3). (F) RNA FISH analysis for circEIF4G3 in GC cells. Scale bar = 25 μm. (G) CircEIF4G3 expression levels in tumor tissues and adjacent non-tumor tissues of patients with GC were detected by qRT-PCR (n = 103). (H) The association between circEIF4G3 expression level and overall survival time was analyzed by Kaplan–Meier plot. Log-rank tests were used to determine statistical significance. (I) CircEIF4G3 expression levels in serum of GC patients, gastritis and healthy controls were detected by qRT-PCR. *P < 0.05; ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598