Skip to main content
Fig. 5 | Molecular Cancer

Fig. 5

From: Circular RNA EIF4G3 suppresses gastric cancer progression through inhibition of β-catenin by promoting δ-catenin ubiquitin degradation and upregulating SIK1

Fig. 5

CircEIF4G3 functions as a miR-4449 sponge. (A) RIP assay was applied using Ago2 antibody in GC cells. The relative RNA level of circEIF4G3 was detected by qRT-PCR. (B) HEK293T cells were transfected with different plasmid or miRNAs as indicated. Relative luciferase activity was detected and normalized to the Renilla internal control. (C) Luciferase reporter assay for luciferase activity of circEIF4G3 in GC cells co-transfected with miRNA mimics. (D-G) Rescue experiments for cell growth curve (D), colony formation (E), transwell migration (F), and matrigel invasion assays (G) in GC cells co-transfected with circEIF4G3 and miR-4449. Data are shown as means ± SD (n = 3). Scale bars = 100 μm. (H) GO analysis and enriched GO terms of differentially expressed genes in GC on their biological process (BP). (I) The expression of indicated proteins in rescue experiment was determined by western blot. *P < 0.01, **P < 0.01, ***P < 0.001

Back to article page

Molecular Cancer

ISSN: 1476-4598