Skip to main content
Fig. 6 | Molecular Cancer

Fig. 6

From: Circular RNA EIF4G3 suppresses gastric cancer progression through inhibition of β-catenin by promoting δ-catenin ubiquitin degradation and upregulating SIK1

Fig. 6

SIK1 is a target of miR-4449 and circEIF4G3 modulates miR-4449/SIK1 axis in GC. (A) Bioinformatic analysis of potential target genes for miR-4449. (B-C) The mRNA expression (B) and protein levels (C) of potential target genes for miR-4449 in GC cells transfected with circEIF4G3 or miRNA mimics was determined by qRT-PCR and Western blot. (D) Relative luciferase activities of wild-type (WT) and mutated (MUT) SIK1 reporter plasmid in GC cells co-transfected with miR-4449 mimics. (E) TCGA data analysis for the expression of miR-4449 in GC tissues. (F) QRT-PCR analysis for the expression of SIK1 in GC tissues. Correlation analysis of circEIF4G3 and SIK1 gene expression in tumor tissues of patients with GC. CircEIF4G3 and si-SIK1 were co-transfected into GC cells. GC cell proliferation (G), migration (H) and invasion (I) were determined. (J) The expression of β-catenin, c-Myc, and cyclin D1 was determined by western blot. Scale bar = 100 μm. Data are shown as means ± SD. *P < 0.01, **P < 0.01, ***P < 0.001

Back to article page

Molecular Cancer

ISSN: 1476-4598