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Fig. 10 | Molecular Cancer

Fig. 10

From: Circular RNA circPOLR2A promotes clear cell renal cell carcinoma progression by facilitating the UBE3C-induced ubiquitination of PEBP1 and, thereby, activating the ERK signaling pathway

Fig. 10

Knockdown of the m6A reader YTHDF2 promoted the expression of circPOLR2A in cRCC cells. a Pull-down assay validated the binding of YTHDF2 and circPOLR2A. Top, 786O cells; bottom, Caki1 cells. b RIP assay confirmed the binding of YTHDF2 and circPOLR2A. Left, 786O cells; right, Caki1 cells. c RNA FISH and immunofluorescence analysis of the colocalization of YTHDF2 (red) and circPOLR2A (green). d YTHDF2 expression was lower in cRCC tissues than in normal tissues based on TCGA database. e Kaplan-Meier method and log-rank test indicated that YTHDF2 expression was a favorable prognostic factor in the TCGA-KIRC cohort. f MeRIP assay verified the enrichment of circPOLR2A in m6A-precipitated fraction. g-h The expression of circPOLR2A in cRCC cells transfected with siNC, siMETTL3 or siYTHDF2. 10 g, 786O; 10 h, Caki1. i MeRIP assay determined the relative enrichment of circPOLR2A-WT or circPOLR2A-MUT in m6A-precipitated fraction in 786O cells transfected with circPOLR2A-WT or circPOLR2A-MUT plasmids. j Analysis of the relative abundance of circPOLR2A-WT or circPOLR2A-MUT in circPOLR2A-WT-expressing or circPOLR2A-MUT-expressing 786O cells transfected with siNC, siMETTL3 or siYTHDF2. k Analysis on the relative abundance of circPOLR2A in cRCC cells transfected with siNC or siYTHDF2. Blue, cRCC cells were pretreated with DMSO; Red, cRCC cells were pretreated with 10 μM DZNep for 1 h

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Molecular Cancer

ISSN: 1476-4598