Skip to main content
Fig. 2 | Molecular Cancer

Fig. 2

From: Circular RNA circPOLR2A promotes clear cell renal cell carcinoma progression by facilitating the UBE3C-induced ubiquitination of PEBP1 and, thereby, activating the ERK signaling pathway

Fig. 2

Characterization of circPOLR2A. a Schematic illustration of circPOLR2A originating from the POLR2A gene on chromosome 17 (top). The backsplice junction site of circPOLR2A was identified via Sanger sequencing (bottom). b The existence of circPOLR2A and POLR2A mRNA in cDNA and gDNA was determined by PCR and electrophoresis analysis. c, d The abundance of circPOLR2A and POLR2A mRNA after RNase R treatment was detected via qRT-PCR. e, f The RNA abundance of circPOLR2A and POLR2A mRNA in cRCC cells treated with actinomycin D was determined at different time points. g Representative FISH images of circPOLR2A in 786O cells. h, i Nuclear and cytoplasmic fractionation revealed the level of circPOLR2A in the nucleus and cytoplasm. U6 and GAPDH were used as positive controls in the nucleus and cytoplasm, respectively

Back to article page

Molecular Cancer

ISSN: 1476-4598