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Fig. 4 | Molecular Cancer

Fig. 4

From: Circular RNA circPOLR2A promotes clear cell renal cell carcinoma progression by facilitating the UBE3C-induced ubiquitination of PEBP1 and, thereby, activating the ERK signaling pathway

Fig. 4

CircPOLR2A could interact with the PEBP1 protein. a RNA pull-down assay was performed in cRCC cells using a circPOLR2A sense probe (circPOLR2A probe) and an antisense probe (ctrl probe). b Venn diagram of the overlapping proteins (including PEBP1 and SMARCA1) between circPOLR2A-interacting proteins identified by MS and proteins with independent prognostic value in the cRCC cohort from the CPTAC database. c The effect of circPOLR2A on the protein levels of PEBP1 and SMARCA1 was assessed by western blotting. d RNA pull-down assay and western blotting validated the existence of PEBP1 in the precipitates pulled down with the circPOLR2A probe. e RIP assay validated the interaction between PEBP1 and circPOLR2A. Top, western blot analysis for IP efficiency of PEBP1 antibody; bottom, the enrichment of circPOLR2A in the precipitates of PEBP1 antibody relative to input group. f RNA FISH and immunofluorescence analysis indicated the colocalization of PEBP1 (red) and circPOLR2A (green) in 786O cells. g Forest plots showing the prognostic value of PEBP1 protein in the cRCC cohort from the CPTAC database. Top, the results of univariate Cox regression analysis (green dots); bottom, the results of multivariate Cox regression analysis (red dots). h GO enrichment analysis of circPOLR2A-interacting proteins identified via RNA pull-down and MS.

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