Skip to main content
Fig. 8 | Molecular Cancer

Fig. 8

From: Circular RNA circPOLR2A promotes clear cell renal cell carcinoma progression by facilitating the UBE3C-induced ubiquitination of PEBP1 and, thereby, activating the ERK signaling pathway

Fig. 8

CircPOLR2A enhanced the UBE3C-mediated ubiquitination of PEBP1. a After circPOLR2A knockdown or overexpression, western blotting detected the PEBP1 protein level in cRCC cells with UBE3C overexpression. Top, 786O cells with circPOLR2A knockdown; bottom, Caki1 cells with ectopic circPOLR2A expression. b, c Ubiquitination modification of PEBP1 in cRCC cells after ectopic UBE3C expression and circPOLR2A knockdown or overexpression detected via Co-IP and western blotting. 8b, 786O cells with circPOLR2A knockdown; 8c, Caki1 cells with ectopic circPOLR2A expression. d, e The binding of PEBP1 and UBE3C in cRCC cells after transfection with circPOLR2A knockdown (8d) or overexpression (8e) and treatment with 10 μg/ml RNase A. f, g Western blotting verified the activation of the ERK pathway in cRCC cells. 8f, 786O cells; 8 g, Caki1 cells. h Tube formation assay evaluated the angiogenesis of HUVECs incubated with the culture medium from cRCC cells transfected with circPOLR2A knockdown or overexpression. Top, 786O cells; bottom, Caki1 cells. Scale bar, 100 μm. i, j Tube formation assay for rescue experiments. 8i, 786O cells; 8j, Caki1 cells. Scale bar, 100 μm. k, l Statistical analysis on total number of junctions in the tube formation assay for rescue experiments

Back to article page

Molecular Cancer

ISSN: 1476-4598