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Fig. 1 | Molecular Cancer

Fig. 1

From: circFBXO7/miR-96-5p/MTSS1 axis is an important regulator in the Wnt signaling pathway in ovarian cancer

Fig. 1

circFBXO7 is downregulated in ovarian cancer and is associated with poor prognosis. A Volcano plot showing the relative expression of circRNA in ovarian cancer and normal ovarian tissue. circFBXO7 is marked as a red circle. Expression of circFBXO7 and its parental gene FBXO7 in tumor and normal tissue is shown in the right panel. B Schematic diagram of the formation of circFBXO7. circFBXO7 was back-spliced from 3 exons of FBXO7 and its full-length sequence was confirmed by Sanger sequencing. C Agarose gel electrophoresis showed circFBXO7 as amplified by divergent primers in cDNA but not in gDNA of A2780 and MDAH2774. D The full length of circFBXO7 obtained by two different primers. E Relative abundance of circFBXO7 and linear-FBXO7 in TOV21G and A2780 cells treated with RNase R, as determined by qRT-PCR. F qRT-PCR analysis of the expression of circFBXO7 in an independent cohort including 12 tumor tissues from ovarian cancer patients and 12 normal tissues from patients with benign gynaecological diseases. G Kaplan-Meier survival curve of circFBXO7 expression level in 70 FFPE tissues from ovarian cancer patients. The expression level of circFBXO7 was evaluated using the BaseScope assay. H BaseScope images (20× magnification) showing the signal points which measure the expression of circFBXO7. The two upper panels represent the better the prognosis with higher the expressions of circFBXO7, and the two lower panels represent the worse the prognosis with lower expressions of circFBXO7. I Kaplan-Meier survival curve of linear FBXO7 expression level of ovarian cancer patients from TCGA. *** P < 0.001, ** P < 0.01, * P < 0.05

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