Fig. 4

MTSS1 is a target of miR-96-5p. A Venn diagram of miR-96-5p targets predicted by miRDB, TarBase and TargetScan. B qRT-PCR analysis of potential miR-96-5p target genes that were predicted by all three miRNA target databases (miRTarBase, TargetScan and miRDB) in A2780 and MDAH2774 cells transfected with circFBXO7 and mock plasmids. C Luciferase report vector of wild-type and mutant MTSS1. D Luciferase activity of MTSS1 wild-type and mutant cells transfected with miR-96-5p mimics and negative control mimics. E Western blot analysis of MTSS1 expression in A2780 and MDAH2774 cells transfected with miR-96-5p mimics or negative control mimics, and miR-96-5p inhibitors or negative control inhibitors. F Western blot analysis of the expression of MTSS1 in A2780 and MDAH2774 cells transfected with circFBXO7 or mock plasmids. *** P < 0.001, ** P < 0.01, * P < 0.05