Fig. 6

circFBXO7 regulates Wnt/β-catenin signaling pathway in ovarian cancer. A Enrichment analysis of signaling pathway after circFBXO7 overexpression using RNA-seq data. B Topflash/Fopflash assays performed in MDAH2774 and A2780 cells with circFBXO7 overexpression plasmid transfection. C Topflash/Fopflash assays performed in MDAH2774 and A2780 cells with MTSS1 siRNA transfection. D Western blot analysis of the expression of Wnt/β-catenin and p-GSK3β in A2780 and MDAH2774 cells transfected with circFBXO7 or mock plasmids, miR-96-5p mimics or negative control mimics, MTSS1 siRNA or negative controls. E Western blot analysis of the expression of Wnt/β-catenin and p-GSK3β in circFBXO7 overexpression A2780 and MDAH2774 cells transfected with miR-96-5p mimics or negative control mimics. F Western blot analysis of the expression of Wnt/β-catenin and p-GSK3β in circFBXO7 overexpression A2780 and MDAH2774 cells transfected with MTSS1 siRNA or negative controls. G Western blot analysis of the expression of Wnt/β-catenin and p-GSK3β in circFBXO7 overexpression A2780 and MDAH2774 cells transfected with miR-96-5p mimics or negative control mimics. H Western blot analysis of the expression of Wnt/β-catenin and p-GSK3β in circFBXO7 overexpression A2780 and MDAH2774 cells transfected with MTSS1 siRNA or negative control siRNA. i Representative images of IF micrographs of the subcellular localization and expression of β-catenin (green) in MDAH2774 cells transfected with circFBXO7 siRNA, MTSS1 siRNA, or negative control siRNA. Nuclei were counterstained with DAPI (blue). LiCl (Lithium chloride) is a Wnt activator which acts directly on GSK3β. *** P < 0.001, ** P < 0.01, * P < 0.05