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Fig. 3 | Molecular Cancer

Fig. 3

From: CircBCAR3 accelerates esophageal cancer tumorigenesis and metastasis via sponging miR-27a-3p

Fig. 3

Silenced circBCAR3 inhibits the ferroptosis of esophageal cancer cells in vitro. A Intracellular Fe2+ levels in EC109 and KYSE150 cell after transfection with circBCAR3 shRNA were detected with an iron assay kit. B MDA level was detected with a lipid peroxidation MDA assay kit. C Lipid ROS levels were assessed using C11-BODIPY581/591 probe. Fluorescence analysis was conducted using flow cytometry. D GSH levels were measured with a Glutathione assay kit. E Transmission electron microscopy was utilized to observe morphological changes in EC109 and KYSE150 cell after transfection with circBCAR3 shRNA. F Western blotting was performed to examine GPX4 protein. * p < 0.05, ** p < 0.01. One-way ANOVA was performed

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