Fig. 5
From: CircBCAR3 accelerates esophageal cancer tumorigenesis and metastasis via sponging miR-27a-3p
Knockdown of circBCAR3 inhibits the esophageal cancer growth and metastasis in vivo. A Photographs of xenograft tumors formed by EC109 cells stably expressing sh-circBCAR3 and sh-NC. B The tumor volume of mice in the sh-circBCAR3 and the control group was shown. C The tumor weight was shown. D Ki-67 immunohistochemistry staining and H&E staining of xenograft tumors. E Xenogen images illustrate the growth of the tumor inside the mice. F The lung tissues with metastasis from mice were shown. G H&E staining was performed to reveal the metastatic foci in lung tissues. H IHC was used to evaluate the expression of EMT markers including Vimentin, N-cadherin, MMP-2, MMP-9, and E-cadherin in the lung tissues. n = 6. ** p < 0.01. Two-way ANOVA was performed for difference comparison in B, while student’s t test was used for C and F