Fig. 7

TNPO1 functions as the target of miR-27a-3p in esophageal cancer cells. A Expression of TNPO1, GCC2, THRB, CASC3 in EC109 cells by transfection with miR-27a-3p mimics was assessed by PCR analysis. B PCR analysis of expression of TNPO1 in six esophageal cancer cells and control Het-1A cell line. C The expression of TNPO1 in 182 esophageal cancer tissues was obtained from the GEPIA database. D Binding site of miR-27a-3p on TNPO1 was obtained from ENCORI database. Complementary bases are linked in vertical line. E Regulation of miR-27a-3p on TNPO1 3’UTR was confirmed with luciferase reporter assays. F PCR revealed TNPO1 expression in EC109 and KYSE150 cells by treatment of miR-27a-3p mimics or inhibitor. G Expression of TNPO1 in hypoxic EC109 and KYSE150 cells was revealed by PCR analysis. H PCR showed the significant high expression of TNPO1 in 45 esophageal carcinoma tissues. * p < 0.05, ** p < 0.01, *** p < 0.001. Student’s t test was performed