Fig. 1

MAGI3 expression is downregulated in CRC of primary and recurrence tissues. a, DEGs correlated with CRC carcinogenesis and recurrence. DEGs of tumor vs. adjacent noncancerous tissues, and recurrence vs. non-recurrence tumors in CRC patients from TCGA and GSE40967 were displayed by the volcano plot. The horizontal gray line represented P = 0.01. The vertical gray lines showed 1.5-fold changes in gene expression. Red dots represented upregulated genes and blue dots represented downregulated genes. b, MAGI3 was one of 17 DEGs identified in 2 independent datasets. The UpSetVenny diagram showed the 17 overlapped DEGs, and the importance of MAGI3 ranked the top one in these DEGs by random forest classifier models analysis. c, The protein level of MAGI3 was also robustly reduced in CRC and recurrence specimens. Representative images of MAGI3 IHC staining in adjacent tissues (top), non-recurrence tumors (middle) and recurrence tumors (bottom) of CRC. Scale bars: 50 μm. Right panels are × 2 magnification of the dashed areas on the left. Dot plot showing the corresponding quantification of MAGI3 H-score. Data were presented as the mean ± SEM, and statistical significance was calculated by Mann–Whitney test. ***P < 0.001. d, patients with low MAGI3 protein had a worse OS and RFS. Kaplan–Meier curves showing comparison of the OS (left) and RFS (right) between MAGI3-low and -high expression groups of CRC patients (P < 0.01, calculated by log-rank test). e, MAGI3 was a potential predictor of postoperative recurrence in CRC patients. The receiver operating characteristic curve with serum carcinoembryonic antigen value (left) and MAGI3 in CRC specimens H-score (right)