Fig. 2

MAGI3 inhibits cell growth and induces cell cycle arrest and apoptosis in CRC cells. a and b, Overexpression of MAGI3 significantly inhibited CRC cell proliferation. Cell proliferation of HT115 or RKO cells, transfected with MAGI3 or pcDNA3.0, were assessed by CCK-8 (a) or colony formation assays (b). c and d, Knockdown of MAGI3 expression significantly enhanced CRC cell proliferation. Cell proliferation of HT29 or SW480 cells, transfected with siMAGI3 or siNC, were assessed by CCK-8 (c) or colony formation assays (d). e and f, MAGI3 induced cell cycle arrest. Flow cytometry assay showed that overexpression of MAGI3 induced cell cycle arrest at G1–S transition (e) and knockdown of MAGI3 promoted cell cycle progression (f). g and h, MAGI3 induced cell apoptosis. Flow cytometry assay showed that overexpression of MAGI3 induced CRC cell apoptosis (g) and knockdown of MAGI3 reduced CRC cell apoptosis (h). Q2-1 shows the late apoptotic cells and Q4-1 shows the early apoptotic cells. Data were presented as the mean ± SEM, 2-way repeated-measures ANOVA with Bonferroni post-test (a and c), 2-tailed, unpaired t test (b, d, e–h) were used to determine statistical significance. *P < 0.05, **P < 0.01, and ***P < 0.001. These experiments (a-h) were repeated three times independently