Fig. 4

Pathway switch between AP-1 and RAS signaling in high risk FLT3-ITD (s3432). (A) UMAP of Dx and Re cells for FLT3-ITD patient s3432 colored by timepoint (top) or cell cluster (bottom). (B) Heatmap displaying the top 10 cluster marker genes. Color represents row normalized expression values. (C) Overrepresented GO terms (category: biological pathway) in cluster 1 (Dx) and 3 (Re). P-values: hypergeometric test (BH-corrected). (D) The expression of genes related to AP-1 transcription factor network and RAS signaling pathway in each timepoint. (E) Calculation of LSC17 score for each cluster, and p-value was calculated using Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001