Fig. 5

Putative LSCs detected in AML1-ETO pair (s914). (A) UMAP of Dx and Re cells for AML1-ETO patient s914, colored by timepoint (top) and cell cluster (bottom). Cells in cluster 6 express ambiguous marker genes, and may be doublets or contaminated by ambient RNA and were discarded (see also Supplemental Fig. 6). (B) Heatmap depicting the top 7 cluster markers. Color represents row normalized expression values. (C) Pseudo-time trajectory colored by timepoint (top) or cell cluster (bottom). (D) Heatmap showing representative genes per cluster. (E) LSC17 score per cluster. * p < 0.05, ** p < 0.01, *** p < 0.001, Student’s t-test. (F) Barplots depicting the relative cell abundance per cell cycle phase (inferred from marker gene expression) for each cell cluster. Arrow: cells in cluster 2 and 3 predominantly reside in the G1 phase